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Objectives: Thyroid is an endocrine gland located below the larynx, the principal thyroid hormones are thyroxin (T4) Tri-iodothyroxine (T3) and thyroid stimulating hormone (TSH). The present study was carried out to attain the impact of gender and seasons on the level of thyroxine (T4), (T3) and (TSH) in individuals free of thyroid disease. Methods: The changes of T3, T4 and TSH hormones in the blood serum for females and males were studied for three years (2014, 2015 and 2016). Hormonal assay was done by using VIDAS by (bio -merieuxsa). Results: The results showed that the significant increase of thyroid hormones TSH in males during winter for three years while in female the clear significantly increased in autumn for years 2014, 2015 and 2016 was shown. Then thyroid hormones in T3 and T4 in females showed height significantly increased in summer and spring for years 2015 and 2016, but the levels of T3 in males showed significantly decreased during summer, spring and winter for 2015 and 2016, and the levels of T4 decreased significantly in winter for three years studied. Conclusions: It is concluded that the gender, seasons and unstable conditions in Iraq such as anxiety especially during 2015 and 2016 have an appreciable effect on the levels T3, T4 and TSH compared with 2014 and other countries.
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